Real Time PCR and End Point Analysis

THE 7900HT
The ABI PRISM® 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences using fluorescent primers and probes (Taqman) or Sybr green. The 7900HT system is capable of running 96 and 384 well plates making it ideally suited to meet the high-throughput requirements of a campus wide core facility. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.

Key Features
Interchangeable formats provide improved throughput and flexibility
Custom Automation Accessory provides 24-hour unattended operation.
Hand-held and integrated bar code readers simplify sample tracking.
Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
Proven assay development guidelines save time and money .

The Service :
The 7900HT instrument is housed in the Core Facility and is available to users on a fee for service basis. Each user must undergo basic training before being allowed access to the instrument. Each user must sign up in advance and supply a valid account number before being allowed access to the instrument.The core facility supplies: access to the instrument, basic training, maintenance, analysis software and consultation.The user supplies: a plate (96 or 384 well) of reactions assembled and sealed, ready for the user to run on the instrument.
User can schedule use of the instrument online. Go to the Login page for instructions.

2. Relative Quantitation of Gene Expression http://docs.appliedbiosystems.com/pebiodocs/04303859.pdf

3. Multiplex PCR with TaqMan VIC probes http://docs.appliedbiosystems.com/pebiodocs/04306236.pdf

4. To order Taqman primers and probes http://www.appliedbiosystems.com/products/productdetail.cfm?prod_id=92

Please contact us for more information or for scheduling a training session.